Hotspot

atpF-atpH

LSC · 14666-15254 · 589 bp

Back to Platanus

Pi 0.0103

MarkerSeek score 54.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 9
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CAGTGACCCAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	58.0	58.0	684-691 bp	1.0	81.5
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	712-719 bp	1.0	80.8
atpF-atpH_p3	CAGTGACCCAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	58.0	58.3	671-678 bp	1.0	79.7
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.9	58.3	699-706 bp	1.0	78.9
atpF-atpH_p5	AGTGACCCAAGGAAACGAAA	ATTTATGGACTGGTCGTGGC	45.0	50.0	57.3	58.0	683-690 bp	1.0	76.0