Hotspot

trnS-GCU-trnG-UCC

LSC · 9388-10650 · 1263 bp

Back to Platanus

Pi 0.00163

MarkerSeek score 50.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1263
variable_sites: 3
pi: 0.00163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AGAAACCGGAAAGAGAGGGA	TCGGAGTATGAATCAAACCGA	50.0	42.9	58.0	56.8	1407-1444 bp	1.0	64.9
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	TCGGAGTATGAATCAAACCGA	45.0	42.9	58.0	56.8	1353-1390 bp	1.0	64.9
trnS-GCU-trnG-UCC_p3	AGAAACCGGAAAGAGAGGGA	TGAATCAAACCGAAAGATCCCT	50.0	40.9	58.0	57.7	1399-1436 bp	1.0	63.8
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGATCCCT	45.0	40.9	58.0	57.7	1345-1382 bp	1.0	63.8
trnS-GCU-trnG-UCC_p5	AGAAACCGGAAAGAGAGGGA	TCGGAGTATGAATCAAACCGAA	50.0	40.9	58.0	57.5	1407-1444 bp	1.0	62.5