Hotspot

trnK-UUU-rps16

LSC · 4488-5594 · 1107 bp

Back to Platanus

Pi 0.00423
MarkerSeek score 45.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1107
variable_sites
7
pi
0.00423
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1231-1255 bp 1.0 77.8
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1230-1254 bp 1.0 77.8
trnK-UUU-rps16_p3 TCCTAGCCGCACTTAAAAGC GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 1246-1270 bp 1.0 77.0
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 1240-1264 bp 1.0 76.9
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.5 58.2 1217-1241 bp 1.0 75.5