Hotspot

trnH-GUG-psbA

LSC · 112-611 · 500 bp

Back to Platanus

Pi 0.00139

MarkerSeek score 48.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 1
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATTGAATGATGGGCGAACGA	CAACCGTGCTAACCTTGGTA	45.0	50.0	58.0	57.8	667-686 bp	1.0	80.8
trnH-GUG-psbA_p2	ATTGAATGATGGGCGAACGA	ATCAACCGTGCTAACCTTGG	45.0	50.0	58.0	58.2	669-688 bp	1.0	80.8
trnH-GUG-psbA_p3	GATCCACTTGGCTACATCCG	CAACCGTGCTAACCTTGGTA	55.0	50.0	58.1	57.8	603-622 bp	1.0	80.3
trnH-GUG-psbA_p4	CAATCCACTGCCTTGATCCA	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	617-636 bp	1.0	80.2
trnH-GUG-psbA_p5	AATCCACTGCCTTGATCCAC	CAACCGTGCTAACCTTGGTA	50.0	50.0	57.9	57.8	616-635 bp	1.0	80.2