Hotspot

ycf1

SSC · 123172-128631 · 5460 bp

Back to Platanthera

Pi 0.0192

MarkerSeek score 51.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5460
variable_sites: 218
pi: 0.0192
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGGTCGAATTAGAACGGGTA	AACAGTTTGGGGAACGGAAA	50.0	45.0	57.2	57.9	355 bp	0.5	52.7
ycf1_p2	CGGTCGAATTAGAACGGGTA	AGTTTGGGGAACGGAAACAA	50.0	45.0	57.2	57.9	352 bp	0.5	52.7
ycf1_p3	CGGTCGAATTAGAACGGGTA	TGGGGAACGGAAACAAAACT	50.0	45.0	57.2	57.9	348 bp	0.5	52.7
ycf1_p4	CGGTCGAATTAGAACGGGTA	TTGGTTAACGGACGGTATGC	50.0	50.0	57.2	58.3	487 bp	0.5	51.5
ycf1_p5	CGGTCGAATTAGAACGGGTA	GTTAACGGACGGTATGCAGA	50.0	50.0	57.2	57.7	484 bp	0.5	51.4