Hotspot

rps16

LSC · 4899-6099 · 1201 bp

Back to Platanthera

Pi 0.0247

MarkerSeek score 65.90

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1201
variable_sites: 63
pi: 0.0247
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TATTTGTTCGAGACGCCGTT	CAAGCGGTAAGGCAATAGGT	45.0	50.0	57.9	58.0	1889-1973 bp	1.0	87.1
rps16_p2	TATTTGTTCGAGACGCCGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.9	58.1	1852-1936 bp	1.0	87.0
rps16_p3	GCACAACTGCGTCAAAACAT	CAAGCGGTAAGGCAATAGGT	45.0	50.0	58.2	58.0	2141-2231 bp	1.0	86.1
rps16_p4	GTTGAGTTAGCAACCCGGAT	CAAGCGGTAAGGCAATAGGT	50.0	50.0	57.9	58.0	2219-2293 bp	0.833333	78.7
rps16_p5	GTTGAGTTAGCAACCCGGAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	2182-2256 bp	0.833333	78.5