Hotspot

rpl32-trnL-UAG

SSC · 112989-113641 · 653 bp

Back to Platanthera

Pi 0.039

MarkerSeek score 70.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 653
variable_sites: 59
pi: 0.039
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CTTTTTGACTGTCCGGTGGA	CCTTGGGTAAAAAGGGCACT	50.0	50.0	58.0	58.0	1289-1348 bp	1.0	86.7
rpl32-trnL-UAG_p2	CTTTTTGACTGTCCGGTGGA	GCAGGTACTAATATCGCGGG	50.0	55.0	58.0	58.0	1346-1405 bp	1.0	86.6
rpl32-trnL-UAG_p3	CTTTTTGACTGTCCGGTGGA	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	1150-1209 bp	1.0	86.5
rpl32-trnL-UAG_p4	CTTTTTGACTGTCCGGTGGA	TGGCGAAACCTTGGGTAAAA	50.0	45.0	58.0	57.9	1297-1356 bp	1.0	86.2
rpl32-trnL-UAG_p5	CTTTTTGACTGTCCGGTGGA	GGGTTGATGCATTTCGGTTG	50.0	50.0	58.0	58.0	1329-1341 bp	0.666667	70.1