Hotspot

ndhF-rpl32

SSC · 112053-112814 · 762 bp

Back to Platanthera

Pi 0.0361

MarkerSeek score 70.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 762
variable_sites: 62
pi: 0.0361
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACACTTGGAAAAGCCCACAT	TCCACCGGACAGTCAAAAAG	45.0	50.0	57.9	58.0	944-970 bp	1.0	87.8
ndhF-rpl32_p2	CATTCCCACGAGTTCCCAAA	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	1278-1304 bp	1.0	85.6
ndhF-rpl32_p3	AACACATTCCCACGAGTTCC	TCCACCGGACAGTCAAAAAG	50.0	50.0	58.1	58.0	1282-1308 bp	1.0	85.6
ndhF-rpl32_p4	ACAGGTCGTGTGAACCAAAA	TCCACCGGACAGTCAAAAAG	45.0	50.0	57.9	58.0	1319-1345 bp	1.0	85.4
ndhF-rpl32_p5	AACAGGTCGTGTGAACCAAA	TCCACCGGACAGTCAAAAAG	45.0	50.0	57.9	58.0	1320-1346 bp	1.0	85.3