Hotspot

rrn4.5-rrn5

IRb · 107209-107421 · 213 bp

Back to Platanthera

Pi 0.0354

MarkerSeek score 71.00

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 213
variable_sites: 15
pi: 0.0354
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	330 bp	1.0	83.0
rrn4.5-rrn5_p2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	328 bp	1.0	83.0
rrn4.5-rrn5_p3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	326 bp	1.0	83.0
rrn4.5-rrn5_p4	CGAGCCGCTTATCATTACGA	AGTATCGTCACCGCAGTAGA	50.0	50.0	58.0	58.0	381 bp	1.0	82.5
rrn4.5-rrn5_p5	CGAGCCGCTTATCATTACGA	TATCGTCACCGCAGTAGAGT	50.0	50.0	58.0	58.0	379 bp	1.0	82.5