Hotspot

psbB-psbT

LSC · 72736-73177 · 442 bp

Back to Platanthera

Pi 0.0286

MarkerSeek score 66.40

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 442
variable_sites: 25
pi: 0.0286
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GTGCCAGAACCTTGTTCAGA	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.0	58.1	844-875 bp	1.0	86.1
psbB-psbT_p2	CGGGCTACTTTGAAATCCGA	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.0	58.1	953-984 bp	1.0	85.6
psbB-psbT_p3	TATGGTGGCGAACTCAATGG	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.0	58.1	1049-1080 bp	1.0	84.9
psbB-psbT_p4	ATTGTGAGAGCCGATGTTCC	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.0	58.1	1127-1158 bp	1.0	84.7
psbB-psbT_p5	GGAATTGTGAGAGCCGATGT	CCCTAGTCGCCATCTCCATA	50.0	55.0	58.0	58.1	1130-1161 bp	1.0	84.7