Hotspot

psaA-ycf3

LSC · 40666-41333 · 668 bp

Back to Platanthera

Pi 0.0194

MarkerSeek score 67.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 24
pi: 0.0194
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	GGACAGGCTTGGTTTGATCA	50.0	50.0	57.9	58.1	860-871 bp	1.0	81.5
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TGGACAGGCTTGGTTTGATC	50.0	50.0	57.9	58.1	861-872 bp	1.0	81.5
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	815-826 bp	1.0	80.7
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	814-825 bp	1.0	80.7
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	851-862 bp	1.0	80.0