Hotspot

trnE-UUC-trnT-GGU

LSC · 29988-30889 · 902 bp

Back to Platanthera

Pi 0.0225

MarkerSeek score 70.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 902
variable_sites: 40
pi: 0.0225
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.8	58.1	923-986 bp	1.0	85.4
trnE-UUC-trnT-GGU_p2	CGTTGCCTCCTTGAAAGAGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.8	58.1	920-983 bp	1.0	85.3
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	901-964 bp	1.0	84.6
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	898-961 bp	1.0	84.5
trnE-UUC-trnT-GGU_p5	CGTTGCCTCCTTGAAAGAGA	AACTTACGCCTTACCATGGC	50.0	50.0	57.8	58.3	936-999 bp	1.0	83.9