Hotspot

rpoB-trnC-GCA

LSC · 25592-26837 · 1246 bp

Back to Platanthera

Pi 0.0198

MarkerSeek score 62.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1246
variable_sites: 50
pi: 0.0198
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCAAATTAAGACCGCGGGTA	TTGGTACCTGTTTGAGCCAC	50.0	50.0	58.0	58.0	1733-1765 bp	1.0	85.2
rpoB-trnC-GCA_p2	CCAAATTAAGACCGCGGGTA	GCCACACGAATAACCCAGAT	50.0	50.0	58.0	58.0	1718-1750 bp	1.0	85.0
rpoB-trnC-GCA_p3	CCCCTACACAGGCAAATTGT	TTGGTACCTGTTTGAGCCAC	50.0	50.0	58.1	58.0	2172-2204 bp	1.0	83.8
rpoB-trnC-GCA_p4	CCCCTACACAGGCAAATTGT	GCCACACGAATAACCCAGAT	50.0	50.0	58.1	58.0	2157-2189 bp	1.0	83.7
rpoB-trnC-GCA_p5	CCAAATTAAGACCGCGGGTA	CCTTACCACTTGGCCATACC	50.0	55.0	58.0	57.9	1533-1534 bp	0.5	59.5