Hotspot

trnR-UCU-atpA

LSC · 9410-9549 · 140 bp

Back to Platanthera

Pi 0.0531

MarkerSeek score 70.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 16
pi: 0.0531
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGCCTTCCAAGCTAACGATG	GCCGGCAAATCAAAATTGGA	50.0	45.0	58.0	57.9	750-759 bp	1.0	86.0
trnR-UCU-atpA_p2	AGCCTTCCAAGCTAACGATG	GGGTCGTGAAGCTTATCCAG	50.0	55.0	58.0	58.1	1042-1051 bp	1.0	84.5
trnR-UCU-atpA_p3	AGCCTTCCAAGCTAACGATG	GGTCGTGAAGCTTATCCAGG	50.0	55.0	58.0	58.1	1041-1050 bp	1.0	84.4
trnR-UCU-atpA_p4	CCTAGCCTTCCAAGCTAACG	GGGTCGTGAAGCTTATCCAG	55.0	55.0	58.1	58.1	1045-1054 bp	1.0	84.2
trnR-UCU-atpA_p5	CCTAGCCTTCCAAGCTAACG	GGTCGTGAAGCTTATCCAGG	55.0	55.0	58.1	58.1	1044-1053 bp	1.0	84.2