Hotspot

psbK-psbI

LSC · 7041-7496 · 456 bp

Back to Platanthera

Pi 0.0291

MarkerSeek score 54.90

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 456
variable_sites: 28
pi: 0.0291
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.9	58.0	820-826 bp	1.0	85.4
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.9	58.0	821-827 bp	1.0	85.4
psbK-psbI_p3	ACCTATTGCCTTACCGCTTG	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	1362-1379 bp	1.0	84.7
psbK-psbI_p4	ACCTATTGCCTTACCGCTTG	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.0	58.0	1363-1380 bp	1.0	84.7
psbK-psbI_p5	ACCTATTGCCTTACCGCTTG	ACGCCCCGGATCATTAGATA	50.0	50.0	58.0	58.1	1116-1135 bp	1.0	83.6