Hotspot

rrn23

IRb · 101583-104396 · 2814 bp

Back to Plantago

Pi 0.208

MarkerSeek score 69.50

Primer available yes

Label rrn23

Feature Metadata

label_name: rrn23
feature_type: rRNA
region: IRb
length_bp: 2814
variable_sites: 1368
pi: 0.208
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23_p1	TCCATCCATTTAGCAGCACC	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.9	57.9	2960-2971 bp	1.0	90.7
rrn23_p2	TCCATCCATTTAGCAGCACC	GCCGTGACCTTCTCTTGAAT	50.0	50.0	57.9	57.9	2985-2996 bp	1.0	90.7
rrn23_p3	CCATCCATTTAGCAGCACCT	AAACTTCTGTCGCTCCATCC	50.0	50.0	57.9	57.9	2959-2970 bp	1.0	90.7
rrn23_p4	CCATCCATTTAGCAGCACCT	GCCGTGACCTTCTCTTGAAT	50.0	50.0	57.9	57.9	2984-2995 bp	1.0	90.7
rrn23_p5	GCACCTTAGGATGGCATAGC	AAACTTCTGTCGCTCCATCC	55.0	50.0	58.5	57.9	2945-2956 bp	1.0	87.4