Hotspot

rps12

IRb · 95303-96095 · 793 bp

Back to Plantago

Pi 0.209

MarkerSeek score 62.70

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: IRb
length_bp: 793
variable_sites: 388
pi: 0.209
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	GCTCTATTTGCCTCTGCCAT	TTTACGCAATCGACCGGATT	50.0	45.0	58.0	58.0	1951-1973 bp	1.0	91.7
rps12_p2	GCTCTATTTGCCTCTGCCAT	GTGGTTGGATCCGCATATGT	50.0	50.0	58.0	58.0	1555-1570 bp	1.0	91.4
rps12_p3	GGGATGCCGCTAATAACCAA	TTTACGCAATCGACCGGATT	50.0	45.0	58.0	58.0	1827-1849 bp	0.978723	90.9
rps12_p4	GCTCTATTTGCCTCTGCCAT	TCTGCACCTACGGGATAGAG	50.0	55.0	58.0	58.0	1694-1705 bp	0.106383	55.9
rps12_p5	GGGATGCCGCTAATAACCAA	TCTGCACCTACGGGATAGAG	50.0	55.0	58.0	58.0	1570-1581 bp	0.085106	55.1