Hotspot

accD

LSC · 56054-57562 · 1509 bp

Back to Plantago

Pi 0.133

MarkerSeek score 74.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1509
variable_sites: 615
pi: 0.133
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTCGCGAGTGGTGAAAGTAG	CGGGATGAGTTGCTAGTGAC	50.0	55.0	57.9	58.1	1828-2004 bp	0.148936	53.8
accD_p2	GTAGTGATTTCGCGAGTGGT	CGGGATGAGTTGCTAGTGAC	50.0	55.0	58.0	58.1	1836-2012 bp	0.085106	51.4
accD_p3	TAGTGATTTCGCGAGTGGTG	CGGGATGAGTTGCTAGTGAC	50.0	55.0	58.0	58.1	1835-2011 bp	0.085106	51.4
accD_p4	GAAACCCCCGCGAATAGTAG	CGGGATGAGTTGCTAGTGAC	55.0	55.0	58.1	58.1	1852-1933 bp	0.06383	45.9
accD_p5	GGGATAACTCAATCGACGGG	CGGGATGAGTTGCTAGTGAC	55.0	55.0	57.8	58.1	2065-2146 bp	0.06383	45.8