Hotspot

infA-rps8

LSC · 77643-78541 · 899 bp

Back to Plantago

Pi 0.199

MarkerSeek score 58.40

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 899
variable_sites: 398
pi: 0.199
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.13
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CTTCTCGGACCCTTAAAGCC	GGAAATCCAAAAGGAACGCG	55.0	50.0	58.0	58.0	1538-2348 bp	0.93617	89.1
infA-rps8_p2	TTCTACGTGCACTCTTACGC	GGAAATCCAAAAGGAACGCG	50.0	50.0	58.0	58.0	1968-2224 bp	0.276596	62.7
infA-rps8_p3	CGTTGGGAAGCGATTCAGTA	GGAAATCCAAAAGGAACGCG	50.0	50.0	58.0	58.0	1462-1665 bp	0.255319	62.0
infA-rps8_p4	CGTTGGGAAGCGATTCAGTA	AACGCGAATTTTTGGTGCAA	50.0	40.0	58.0	58.1	1448-1651 bp	0.255319	61.6
infA-rps8_p5	GTGCACTCTTACGCGAACTA	GGAAATCCAAAAGGAACGCG	50.0	50.0	58.0	58.0	1427-2218 bp	0.085106	53.5