Hotspot

psbM-trnD-GUC

LSC · 29551-30087 · 537 bp

Back to Plantago

Pi 0.0441

MarkerSeek score 74.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 158
pi: 0.0441
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ACTCGTAGTGTGGGGAAGAA	CCTGGGGGTAGGGTACTATG	50.0	60.0	58.0	58.0	1526-1594 bp	0.148936	54.6
psbM-trnD-GUC_p2	TCGTAGTGTGGGGAAGAAGT	CCTGGGGGTAGGGTACTATG	50.0	60.0	58.0	58.0	1524-1592 bp	0.148936	54.6
psbM-trnD-GUC_p3	CGTAGTGTGGGGAAGAAGTG	CCTGGGGGTAGGGTACTATG	55.0	60.0	57.9	58.0	1523-1591 bp	0.148936	54.0
psbM-trnD-GUC_p4	ACGAAATGGAAACCCCCTTC	CCTGGGGGTAGGGTACTATG	50.0	60.0	58.1	58.0	1080-1090 bp	0.06383	43.2
psbM-trnD-GUC_p5	CGAAATGGAAACCCCCTTCA	CCTGGGGGTAGGGTACTATG	50.0	60.0	58.1	58.0	1079-1089 bp	0.06383	43.2