Hotspot

rpoB-trnC-GCA

LSC · 26855-28107 · 1253 bp

Back to Plantago

Pi 0.0542

MarkerSeek score 78.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1253
variable_sites: 350
pi: 0.0542
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TTGTTTCCTACTCACGCGAG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1705-1851 bp	1.0	90.9
rpoB-trnC-GCA_p2	TTGTTTCCTACTCACGCGAG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	2268-2481 bp	1.0	90.3
rpoB-trnC-GCA_p3	TTGTTTCCTACTCACGCGAG	CCTCTTTTTCTCGCGGGTAA	50.0	50.0	57.9	57.9	1828-1948 bp	0.659574	77.2
rpoB-trnC-GCA_p4	TTGTTTCCTACTCACGCGAG	GAGATTCTCGCGAGTCCATC	50.0	55.0	57.9	58.0	2126-2135 bp	0.085106	51.7
rpoB-trnC-GCA_p5	TTGTTTCCTACTCACGCGAG	GCGGGTAATCATTCTACGGG	50.0	55.0	57.9	58.2	1894-1904 bp	0.06383	42.9