primer_id label_name rank fwd_seq rev_seq fwd_len rev_len fwd_gc rev_gc fwd_tm rev_tm fwd_self_any_th rev_self_any_th primer3_penalty target_start target_end amplicon_size cross_species_success_rate primer_score rpoB-trnC-GCA_p1 rpoB-trnC-GCA 1 TTGTTTCCTACTCACGCGAG CCGGATTTGAACTGGGGAAA 20 20 50.0 50.0 57.9 58.1 15.9 0.0 0.142454 26855 28107 1705-1851 1.000000 90.9 rpoB-trnC-GCA_p2 rpoB-trnC-GCA 2 TTGTTTCCTACTCACGCGAG AGCCCAAGCGAGACTTACTA 20 20 50.0 50.0 57.9 58.2 15.9 0.0 0.211674 26855 28107 2268-2481 1.000000 90.3 rpoB-trnC-GCA_p3 rpoB-trnC-GCA 3 TTGTTTCCTACTCACGCGAG CCTCTTTTTCTCGCGGGTAA 20 20 50.0 50.0 57.9 57.9 15.9 5.6 0.149275 26855 28107 1828-1948 0.659574 77.2 rpoB-trnC-GCA_p4 rpoB-trnC-GCA 4 TTGTTTCCTACTCACGCGAG GAGATTCTCGCGAGTCCATC 20 20 50.0 55.0 57.9 58.0 15.9 33.3 0.105214 26855 28107 2126-2135 0.085106 51.7 rpoB-trnC-GCA_p5 rpoB-trnC-GCA 5 TTGTTTCCTACTCACGCGAG GCGGGTAATCATTCTACGGG 20 20 50.0 55.0 57.9 58.2 15.9 0.0 0.268320 26855 28107 1894-1904 0.063830 42.9 psbM-trnD-GUC_p1 psbM-trnD-GUC 1 ACTCGTAGTGTGGGGAAGAA CCTGGGGGTAGGGTACTATG 20 20 50.0 60.0 58.0 58.0 0.0 0.0 0.031006 29551 30087 1526-1594 0.148936 54.6 psbM-trnD-GUC_p2 psbM-trnD-GUC 2 TCGTAGTGTGGGGAAGAAGT CCTGGGGGTAGGGTACTATG 20 20 50.0 60.0 58.0 58.0 0.0 0.0 0.031006 29551 30087 1524-1592 0.148936 54.6 psbM-trnD-GUC_p3 psbM-trnD-GUC 3 CGTAGTGTGGGGAAGAAGTG CCTGGGGGTAGGGTACTATG 20 20 55.0 60.0 57.9 58.0 0.0 0.0 0.105922 29551 30087 1523-1591 0.148936 54.0 psbM-trnD-GUC_p4 psbM-trnD-GUC 4 ACGAAATGGAAACCCCCTTC CCTGGGGGTAGGGTACTATG 20 20 50.0 60.0 58.1 58.0 0.0 0.0 0.106395 29551 30087 1080-1090 0.063830 43.2 psbM-trnD-GUC_p5 psbM-trnD-GUC 5 CGAAATGGAAACCCCCTTCA CCTGGGGGTAGGGTACTATG 20 20 50.0 60.0 58.1 58.0 0.0 0.0 0.107764 29551 30087 1079-1089 0.063830 43.2 trnT-GGU-psbD_p1 trnT-GGU-psbD 1 CATAGTACCCTACCCCCAGG AGCTCGAATTGGCGTAACAT 20 20 60.0 45.0 58.0 58.0 0.0 0.0 0.049811 31150 32191 2137-2698 0.978723 90.8 trnT-GGU-psbD_p2 trnT-GGU-psbD 2 CATAGTACCCTACCCCCAGG AAAGGTTGTACCCGTGAACC 20 20 60.0 50.0 58.0 58.0 0.0 4.3 0.054155 31150 32191 1904-2465 0.978723 90.7 trnT-GGU-psbD_p3 trnT-GGU-psbD 3 CATAGTACCCTACCCCCAGG CCAGACTGCCCTAGTGGATA 20 20 60.0 55.0 58.0 57.9 0.0 0.0 0.113740 31150 32191 2239-2800 0.978723 90.2 trnT-GGU-psbD_p4 trnT-GGU-psbD 4 CATAGTACCCTACCCCCAGG GCTCGAATTGGCGTAACATG 20 20 60.0 50.0 58.0 57.9 0.0 0.0 0.116787 31150 32191 2136-2697 0.978723 90.2 trnT-GGU-psbD_p5 trnT-GGU-psbD 5 CATAGTACCCTACCCCCAGG AAAGCGCGGAATGTATTTGC 20 20 60.0 45.0 58.0 58.1 0.0 8.8 0.117570 31150 32191 2446-2993 0.957447 89.4 trnT-UGU-trnL-UAA_p1 trnT-UGU-trnL-UAA 1 CGAAGCCATACCCAATCGAA ACGCAGTCAACTCCATTTGT 20 20 50.0 45.0 58.1 58.0 0.0 0.0 0.084577 45498 46209 1592-1780 0.936170 88.8 trnT-UGU-trnL-UAA_p2 trnT-UGU-trnL-UAA 2 TTGACCTGTTGAACCCTTGG ACGCAGTCAACTCCATTTGT 20 20 50.0 45.0 57.9 58.0 0.0 0.0 0.098580 45498 46209 1529-1717 0.936170 88.7 trnT-UGU-trnL-UAA_p3 trnT-UGU-trnL-UAA 3 CGAAGCCATACCCAATCGAA AGTCATTTCCGCCCCAATAC 20 20 50.0 50.0 58.1 57.9 0.0 0.0 0.104678 45498 46209 2080-2256 0.319149 63.9 trnT-UGU-trnL-UAA_p4 trnT-UGU-trnL-UAA 4 CGAAGCCATACCCAATCGAA AAAGTCATTTCCGCCCCAAT 20 20 50.0 45.0 58.1 58.1 0.0 0.0 0.114705 45498 46209 2082-2258 0.319149 63.8 trnT-UGU-trnL-UAA_p5 trnT-UGU-trnL-UAA 5 TTGACCTGTTGAACCCTTGG AGTCATTTCCGCCCCAATAC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.118681 45498 46209 2017-2193 0.319149 63.8 rbcL-accD_p1 rbcL-accD 1 ACTACAGTTCGGTGGAGGAA ACACCAGGTCTTCGTCCATA 20 20 50.0 50.0 58.0 58.1 0.0 0.0 0.072349 54932 56053 1319-1507 0.297872 63.3 rbcL-accD_p2 rbcL-accD 2 ACTACAGTTCGGTGGAGGAA CAATTGACTAACCTCCCCCG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.043757 54932 56053 1929-2056 0.148936 56.1 rbcL-accD_p3 rbcL-accD 3 ACTACAGTTCGGTGGAGGAA ACTAACCTCCCCCGTGTTTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.033932 54932 56053 1923-1960 0.063830 45.2 rbcL-accD_p4 rbcL-accD 4 GGAGTCAGGAATTAGCTGCC ACTAACCTCCCCCGTGTTTA 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.068145 54932 56053 1768-1805 0.063830 45.1 rbcL-accD_p5 rbcL-accD 5 AATGCTACTGCGGGTACATG ACTAACCTCCCCCGTGTTTA 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.075003 54932 56053 2399-2436 0.063830 44.3 accD_p1 accD 1 TTCGCGAGTGGTGAAAGTAG CGGGATGAGTTGCTAGTGAC 20 20 50.0 55.0 57.9 58.1 9.6 0.0 0.132431 56054 57562 1828-2004 0.148936 53.8 accD_p2 accD 2 GTAGTGATTTCGCGAGTGGT CGGGATGAGTTGCTAGTGAC 20 20 50.0 55.0 58.0 58.1 9.6 0.0 0.079935 56054 57562 1836-2012 0.085106 51.4 accD_p3 accD 3 TAGTGATTTCGCGAGTGGTG CGGGATGAGTTGCTAGTGAC 20 20 50.0 55.0 58.0 58.1 9.6 0.0 0.081306 56054 57562 1835-2011 0.085106 51.4 accD_p4 accD 4 GAAACCCCCGCGAATAGTAG CGGGATGAGTTGCTAGTGAC 20 20 55.0 55.0 58.1 58.1 8.2 0.0 0.210134 56054 57562 1852-1933 0.063830 45.9 accD_p5 accD 5 GGGATAACTCAATCGACGGG CGGGATGAGTTGCTAGTGAC 20 20 55.0 55.0 57.8 58.1 0.0 0.0 0.222502 56054 57562 2065-2146 0.063830 45.8 petA-psbJ_p1 petA-psbJ 1 TGGGATCTGTTGTTTTGGCA CCGGATTAGGTTCATCTCTGT 20 21 45.0 47.6 57.9 56.9 0.0 0.0 2.185016 61579 62626 942-1157 1.000000 74.5 petA-psbJ_p2 petA-psbJ 2 TGGGATCTGTTGTTTTGGCA TCCGGATTAGGTTCATCTCTGT 20 22 45.0 45.5 57.9 58.4 0.0 0.0 2.435932 61579 62626 943-1158 1.000000 72.5 petA-psbJ_p3 petA-psbJ 3 TGGGATCTGTTGTTTTGGCA TCCGGATTAGGTTCATCTCTG 20 21 45.0 47.6 57.9 56.6 0.0 0.0 2.462217 61579 62626 943-1158 1.000000 72.3 petA-psbJ_p4 petA-psbJ 4 GGGATCTGTTGTTTTGGCAC CCGGATTAGGTTCATCTCTGT 20 21 50.0 47.6 57.6 56.9 0.0 0.0 2.544715 61579 62626 941-1156 1.000000 71.6 petA-psbJ_p5 petA-psbJ 5 TGGGATCTGTTGTTTTGGCA TTCCGGATTAGGTTCATCTCTG 20 22 45.0 45.5 57.9 57.3 0.0 4.1 2.751757 61579 62626 944-1159 1.000000 70.0 psbE-petL_p1 psbE-petL 1 AAAGGATCAAAACGGCCAGT AAGCGCTCTTAGTTCAGCTC 20 20 45.0 50.0 58.0 58.0 2.2 12.1 0.007820 63409 64193 1450-1744 1.000000 91.9 psbE-petL_p2 psbE-petL 2 AAGGATCAAAACGGCCAGTT AAGCGCTCTTAGTTCAGCTC 20 20 45.0 50.0 58.0 58.0 2.2 12.1 0.007820 63409 64193 1449-1743 1.000000 91.9 psbE-petL_p3 psbE-petL 3 AAAGGATCAAAACGGCCAGT AGAACGTGGGTCTCCAAAAC 20 20 45.0 50.0 58.0 58.0 2.2 0.0 0.041452 63409 64193 1427-1721 1.000000 91.7 psbE-petL_p4 psbE-petL 4 AAGGATCAAAACGGCCAGTT AGAACGTGGGTCTCCAAAAC 20 20 45.0 50.0 58.0 58.0 2.2 0.0 0.041452 63409 64193 1426-1720 1.000000 91.7 psbE-petL_p5 psbE-petL 5 TAAGCCGTGAATAGCCAACC AAGCGCTCTTAGTTCAGCTC 20 20 50.0 50.0 58.0 58.0 0.0 12.1 0.035877 63409 64193 1558-1852 0.957447 90.0 clpP_p1 clpP 1 TAGCTGACCCTCATAGTCCG GAGTAAATGCGTCTGTCCGT 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.025170 68355 69723 2088-2772 0.234043 61.2 clpP_p2 clpP 2 ATCCCCAACCGCATATTTCC GAGTAAATGCGTCTGTCCGT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.012454 68355 69723 1559-1582 0.106383 51.9 clpP_p3 clpP 3 ATCCCCAACCGCATATTTCC TGAGTAAATGCGTCTGTCCG 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.013825 68355 69723 1583-1583 0.042553 41.6 clpP_p4 clpP 4 TAGCTGACCCTCATAGTCCG TGAGTAAATGCGTCTGTCCG 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.026541 68355 69723 2113-2113 0.042553 41.5 clpP_p5 clpP 5 ATCCCCAACCGCATATTTCC CTGTAATACTCCAACCGCCC 20 20 50.0 55.0 58.0 58.1 0.0 0.0 0.055158 68355 69723 2049-2049 0.042553 41.3 infA-rps8_p1 infA-rps8 1 CTTCTCGGACCCTTAAAGCC GGAAATCCAAAAGGAACGCG 20 20 55.0 50.0 58.0 58.0 0.0 3.5 0.048400 77643 78541 1538-2348 0.936170 89.1 infA-rps8_p2 infA-rps8 2 TTCTACGTGCACTCTTACGC GGAAATCCAAAAGGAACGCG 20 20 50.0 50.0 58.0 58.0 16.2 3.5 0.049673 77643 78541 1968-2224 0.276596 62.7 infA-rps8_p3 infA-rps8 3 CGTTGGGAAGCGATTCAGTA GGAAATCCAAAAGGAACGCG 20 20 50.0 50.0 58.0 58.0 16.2 3.5 0.027394 77643 78541 1462-1665 0.255319 62.0 infA-rps8_p4 infA-rps8 4 CGTTGGGAAGCGATTCAGTA AACGCGAATTTTTGGTGCAA 20 20 50.0 40.0 58.0 58.1 16.2 15.9 0.076688 77643 78541 1448-1651 0.255319 61.6 infA-rps8_p5 infA-rps8 5 GTGCACTCTTACGCGAACTA GGAAATCCAAAAGGAACGCG 20 20 50.0 50.0 58.0 58.0 15.9 3.5 0.049673 77643 78541 1427-2218 0.085106 53.5 rps12_p1 rps12 1 GCTCTATTTGCCTCTGCCAT TTTACGCAATCGACCGGATT 20 20 50.0 45.0 58.0 58.0 0.0 9.5 0.034482 95303 96095 1951-1973 1.000000 91.7 rps12_p2 rps12 2 GCTCTATTTGCCTCTGCCAT GTGGTTGGATCCGCATATGT 20 20 50.0 50.0 58.0 58.0 0.0 7.4 0.054227 95303 96095 1555-1570 1.000000 91.4 rps12_p3 rps12 3 GGGATGCCGCTAATAACCAA TTTACGCAATCGACCGGATT 20 20 50.0 45.0 58.0 58.0 0.0 9.5 0.037277 95303 96095 1827-1849 0.978723 90.9 rps12_p4 rps12 4 GCTCTATTTGCCTCTGCCAT TCTGCACCTACGGGATAGAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.038885 95303 96095 1694-1705 0.106383 55.9 rps12_p5 rps12 5 GGGATGCCGCTAATAACCAA TCTGCACCTACGGGATAGAG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.041680 95303 96095 1570-1581 0.085106 55.1 rrn16_p1 rrn16 1 GAAGACCCGGCTCTAAGTTG GGACGTAACAAAGGGGATCC 20 20 55.0 55.0 58.0 58.0 0.0 1.9 0.045644 97790 99280 2071-2087 1.000000 91.6 rrn16_p2 rrn16 2 GAAGACCCGGCTCTAAGTTG GGGACGTAACAAAGGGGATC 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.045644 97790 99280 2072-2088 1.000000 91.6 rrn16_p3 rrn16 3 GAAGACCCGGCTCTAAGTTG GGCAAACCAAAAGACCCAAC 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.063436 97790 99280 1915-1931 1.000000 91.5 rrn16_p4 rrn16 4 GAAGACCCGGCTCTAAGTTG AGACGTAGCTCCCCTCTTTT 20 20 55.0 50.0 58.0 58.1 0.0 0.0 0.071560 97790 99280 1955-1971 1.000000 91.4 rrn16_p5 rrn16 5 GAAGACCCGGCTCTAAGTTG ACGTTTCACTCAGACGTAGC 20 20 55.0 50.0 58.0 58.0 0.0 0.0 0.059219 97790 99280 1966-1982 0.404255 67.7 rrn23_p1 rrn23 1 TCCATCCATTTAGCAGCACC AAACTTCTGTCGCTCCATCC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.157742 101583 104396 2960-2971 1.000000 90.7 rrn23_p2 rrn23 2 TCCATCCATTTAGCAGCACC GCCGTGACCTTCTCTTGAAT 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.157742 101583 104396 2985-2996 1.000000 90.7 rrn23_p3 rrn23 3 CCATCCATTTAGCAGCACCT AAACTTCTGTCGCTCCATCC 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.163575 101583 104396 2959-2970 1.000000 90.7 rrn23_p4 rrn23 4 CCATCCATTTAGCAGCACCT GCCGTGACCTTCTCTTGAAT 20 20 50.0 50.0 57.9 57.9 0.0 0.0 0.163575 101583 104396 2984-2995 1.000000 90.7 rrn23_p5 rrn23 5 GCACCTTAGGATGGCATAGC AAACTTCTGTCGCTCCATCC 20 20 55.0 50.0 58.5 57.9 4.8 0.0 0.572475 101583 104396 2945-2956 1.000000 87.4 ndhF_p1 ndhF 1 AATACCGTTCCCCATGCAAA CGGCGCTTTGCTAATTCATT 20 20 45.0 45.0 57.8 57.8 0.0 7.6 0.417110 107115 109346 2923-2995 0.063830 46.7 ndhF_p2 ndhF 2 TACCGTTCCCCATGCAAATC CGGCGCTTTGCTAATTCATT 20 20 50.0 45.0 58.2 57.8 0.0 7.6 0.435624 107115 109346 2921-2993 0.063830 46.5 ndhF_p3 ndhF 3 GCCACAGCTGATGAAGATGA CGGCGCTTTGCTAATTCATT 20 20 50.0 45.0 58.0 57.8 23.3 7.6 0.227410 107115 109346 2877-2981 0.063830 42.1 ndhF_p4 ndhF 4 TTTTCCTGTGCCTACGCTTT CGGCGCTTTGCTAATTCATT 20 20 45.0 45.0 58.0 57.8 0.0 7.6 0.217646 107115 109346 2949-2950 0.042553 40.0 ndhF_p5 ndhF 5 TTTTTCCTGTGCCTACGCTT CGGCGCTTTGCTAATTCATT 20 20 45.0 45.0 58.0 57.8 0.0 7.6 0.217646 107115 109346 2950-2951 0.042553 40.0 ndhF-rpl32_p1 ndhF-rpl32 1 TCGTTGCTGTCGGAAAAAGA TTGATTCCAACCCTTCAGGC 20 20 45.0 50.0 58.1 58.1 0.0 0.0 0.133967 109347 110033 975-976 0.042553 40.7 ndhF-rpl32_p2 ndhF-rpl32 2 GCCCACATACGACGAAGATT TTGATTCCAACCCTTCAGGC 20 20 50.0 50.0 58.1 58.1 0.0 0.0 0.140712 109347 110033 997-998 0.042553 40.7 ndhF-rpl32_p3 ndhF-rpl32 3 ACTCGGAAAAGCCCACATAC TTGATTCCAACCCTTCAGGC 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.186069 109347 110033 1007-1008 0.042553 40.3 ndhF-rpl32_p4 ndhF-rpl32 4 TCTCAAGTAGCCCTGATCGT TTGATTCCAACCCTTCAGGC 20 20 50.0 50.0 57.9 58.1 0.0 0.0 0.222041 109347 110033 1244-1245 0.042553 40.0 ndhF-rpl32_p5 ndhF-rpl32 5 TCAAGTAGCCCTGATCGTGA TTGATTCCAACCCTTCAGGC 20 20 50.0 50.0 58.2 58.1 0.0 0.0 0.229316 109347 110033 1242-1243 0.042553 39.9 rpl32-trnL-UAG_p1 rpl32-trnL-UAG 1 AAAAGGAAGGGGTATTGGGC GCCCCTTTCGGATGAATCAT 20 20 50.0 50.0 57.8 58.0 0.0 0.0 0.260817 110199 110804 939-1238 0.914894 86.5 rpl32-trnL-UAG_p2 rpl32-trnL-UAG 2 AAAAGCGTTTTCCTTGGGGA ATGCCCCTTTCGGATGAATC 20 20 45.0 50.0 58.2 58.0 0.0 0.0 0.230046 110199 110804 993-1214 0.382979 65.5 rpl32-trnL-UAG_p3 rpl32-trnL-UAG 3 AAAAGCGTTTTCCTTGGGGA GCCCCTTTCGGATGAATCAT 20 20 45.0 50.0 58.2 58.0 0.0 0.0 0.230046 110199 110804 991-1212 0.340426 63.8 rpl32-trnL-UAG_p4 rpl32-trnL-UAG 4 AATGAATTAGCAAAGCGCCG GCCCCTTTCGGATGAATCAT 20 20 45.0 50.0 57.8 58.0 2.3 0.0 0.206424 110199 110804 1168-1232 0.127660 54.3 rpl32-trnL-UAG_p5 rpl32-trnL-UAG 5 AATGAATTAGCAAAGCGCCG ATGCCCCTTTCGGATGAATC 20 20 45.0 50.0 57.8 58.0 2.3 0.0 0.206424 110199 110804 1170-1234 0.127660 54.2 rps7_p1 rps7 1 CCCTAGATGCTGTCGGAGTA CTCTCCATCGGAACAACAGG 20 20 55.0 55.0 58.0 58.0 0.0 0.0 0.033076 137068 137535 1636-1648 0.893617 87.5 rps7_p2 rps7 2 AGTTTCGTAAGGGGACTGGA CTCTCCATCGGAACAACAGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.035543 137068 137535 1496-1508 0.893617 87.5 rps7_p3 rps7 3 GCGTTCTAGTGCGTTGTAGA CTCTCCATCGGAACAACAGG 20 20 50.0 55.0 58.0 58.0 9.6 0.0 0.042598 137068 137535 1599-1611 0.893617 87.4 rps7_p4 rps7 4 TTTCCCTGACTTTGTCCGTG CTCTCCATCGGAACAACAGG 20 20 50.0 55.0 58.0 58.0 0.0 0.0 0.053942 137068 137535 1381-1393 0.893617 87.3 rps7_p5 rps7 5 CCTGACTTTGTCCGTGTCAA CTCTCCATCGGAACAACAGG 20 20 50.0 55.0 58.1 58.0 0.0 0.0 0.067632 137068 137535 1377-1389 0.893617 87.2 ndhB_p1 ndhB 1 ATGGCAGAGGCAAATAGAGC GGACGACGTAGGGATCCATA 20 20 50.0 55.0 58.0 58.1 0.0 15.2 0.119955 137865 140026 2764-2773 0.361702 65.5 ndhB_p2 ndhB 2 TTGGTTATTAGCGGCATCCC GGACGACGTAGGGATCCATA 20 20 50.0 55.0 58.0 58.1 1.4 15.2 0.122749 137865 140026 2888-2897 0.361702 65.5 ndhB_p3 ndhB 3 GGCAGAGGCAAATAGAGCTT GGACGACGTAGGGATCCATA 20 20 50.0 55.0 58.0 58.1 0.0 15.2 0.148297 137865 140026 2762-2771 0.361702 65.3 ndhB_p4 ndhB 4 ATGGCAGAGGCAAATAGAGC TCGGTCATATAAGCCCGAGT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.030425 137865 140026 2944-2992 0.191489 49.3 ndhB_p5 ndhB 5 GGCAGAGGCAAATAGAGCTT TCGGTCATATAAGCCCGAGT 20 20 50.0 50.0 58.0 58.0 0.0 0.0 0.058767 137865 140026 2942-2990 0.191489 49.1