Hotspot

rpl22-rps19

LSC · 85640-85719 · 80 bp

Back to Plagiopetalum

Pi 0.025

MarkerSeek score 66.90

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 80
variable_sites: 2
pi: 0.025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCACCTTGTGAGCAGATAGT	TCAATTTCCGTGGACATGCA	50.0	45.0	57.6	58.1	221 bp	1.0	76.8
rpl22-rps19_p2	GCACCTTGTGAGCAGATAGT	ACAGATCGTATGGTCGGTCA	50.0	50.0	57.6	58.2	267 bp	1.0	75.5
rpl22-rps19_p3	CGCACCTTGTGAGCAGATAG	TCAATTTCCGTGGACATGCA	55.0	45.0	58.7	58.1	222 bp	1.0	74.2
rpl22-rps19_p4	TACTCTTCGCACCTTGTGAG	TCAATTTCCGTGGACATGCA	50.0	45.0	57.3	58.1	229 bp	1.0	74.1
rpl22-rps19_p5	CGCACCTTGTGAGCAGATAG	ACAGATCGTATGGTCGGTCA	55.0	50.0	58.7	58.2	268 bp	1.0	72.9