Hotspot

rpl20-rps12

LSC · 70339-71175 · 837 bp

Back to Plagiopetalum

Pi 0.00358
MarkerSeek score 60.80
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
837
variable_sites
3
pi
0.00358
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 CTCGAATGAGCTCCACGAAA AAATGTCACGAAATCCCCCG 50.0 50.0 58.0 58.5 993 bp 1.0 75.9
rpl20-rps12_p2 TCGAATGAGCTCCACGAAAG AAATGTCACGAAATCCCCCG 50.0 50.0 58.0 58.5 992 bp 1.0 75.9
rpl20-rps12_p3 CACGAAAGCTTGACGCAAAT AAATGTCACGAAATCCCCCG 45.0 50.0 58.0 58.5 980 bp 1.0 75.6
rpl20-rps12_p4 CTCGAATGAGCTCCACGAAA CAGCGACGAGGAACATGTAC 50.0 55.0 58.0 58.7 956 bp 1.0 74.4
rpl20-rps12_p5 TCGAATGAGCTCCACGAAAG CAGCGACGAGGAACATGTAC 50.0 55.0 58.0 58.7 955 bp 1.0 74.4