Hotspot

trnM-CAU-atpE

LSC · 53406-53567 · 162 bp

Back to Plagiopetalum

Pi 0.0248
MarkerSeek score 67.40
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
162
variable_sites
4
pi
0.0248
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 TGAGTTCCTTTGCGTTTGGT GAGGCTATCAATGCGGGTTT 45.0 50.0 58.2 58.3 319-320 bp 1.0 76.8
trnM-CAU-atpE_p2 TATTGCTTTCATACGGCGGG GAGGCTATCAATGCGGGTTT 50.0 50.0 58.4 58.3 255-256 bp 1.0 75.7
trnM-CAU-atpE_p3 TGAGTTCCTTTGCGTTTGGT ACACGAGTCGAGGCTATCAA 45.0 50.0 58.2 58.5 328-329 bp 1.0 75.0
trnM-CAU-atpE_p4 TGAGTTCCTTTGCGTTTGGT CGAGTCGAGGCTATCAATGC 45.0 55.0 58.2 58.6 325-326 bp 1.0 74.6
trnM-CAU-atpE_p5 TGAGTTCCTTTGCGTTTGGT GAGTCGAGGCTATCAATGCG 45.0 55.0 58.2 58.6 324-325 bp 1.0 74.6