Hotspot

trnV-UAC-trnM-CAU

LSC · 53133-53333 · 201 bp

Back to Plagiopetalum

Pi 0.00498
MarkerSeek score 47.00
Primer available yes
Label trnV-UAC-trnM-CAU

Feature Metadata

label_name
trnV-UAC-trnM-CAU
feature_type
igs
region
LSC
length_bp
201
variable_sites
1
pi
0.00498
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnM-CAU_p1 CATGTGATGGACCCCTTTGA CCCGCCGTATGAAAGCAATA 50.0 50.0 57.5 58.4 318 bp 1.0 72.9
trnV-UAC-trnM-CAU_p2 TCATGTGATGGACCCCTTTG CCCGCCGTATGAAAGCAATA 50.0 50.0 57.5 58.4 319 bp 1.0 72.9
trnV-UAC-trnM-CAU_p3 CGTGTAAACGAGGTGCTCTA CCCGCCGTATGAAAGCAATA 50.0 50.0 57.4 58.4 284 bp 1.0 72.0
trnV-UAC-trnM-CAU_p4 CATCATGTGATGGACCCCTT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.3 58.4 321 bp 1.0 71.0
trnV-UAC-trnM-CAU_p5 CATGTGATGGACCCCTTTGA CCGCCGTATGAAAGCAATAC 50.0 50.0 57.5 57.3 317 bp 1.0 70.8