Hotspot

trnE-UUC-trnT-GGU

LSC · 31496-32392 · 897 bp

Back to Plagiopetalum

Pi 0.0101

MarkerSeek score 58.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 897
variable_sites: 9
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	979-1045 bp	1.0	78.9
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	958-1024 bp	1.0	78.4
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	955-1021 bp	1.0	78.3
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	976-1042 bp	1.0	77.7
trnE-UUC-trnT-GGU_p5	TTTTCGGAGTACCCTACCCC	GAACCGATGACTTACGCCTT	55.0	50.0	58.4	58.0	1039-1105 bp	1.0	77.6