Hotspot

trnC-GCA-petN

LSC · 28599-29454 · 856 bp

Back to Plagiopetalum

Pi 0.00467

MarkerSeek score 61.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 856
variable_sites: 4
pi: 0.00467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	919 bp	1.0	79.6
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CCTTTAGAGTCCACTTCGCC	50.0	55.0	58.1	58.0	978 bp	1.0	79.6
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CTTTAGAGTCCACTTCGCCC	50.0	55.0	58.1	58.0	977 bp	1.0	79.6
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	913 bp	1.0	78.2
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	AGACGACCATTAAAGCAGCC	50.0	50.0	58.1	58.3	928 bp	1.0	77.6