Hotspot

ycf66

Genome · 80948-81932 · 985 bp

Back to Plagiomnium

Pi 0.00102

MarkerSeek score 59.30

Primer available yes

Label ycf66

Feature Metadata

label_name: ycf66
feature_type: gene
region: Genome
length_bp: 985
variable_sites: 1
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	AAGTGGTAAGGCAGAGGACT	AGGCCTTGATGGTGAAATGG	50.0	50.0	58.0	58.1	2125 bp	1.0	78.6
ycf66_p2	TTGGAAAGAGGCGATATGGC	AGGCCTTGATGGTGAAATGG	50.0	50.0	58.0	58.1	2146 bp	1.0	78.6
ycf66_p3	GCGATATGGCCAAGTGGTAA	AGGCCTTGATGGTGAAATGG	50.0	50.0	58.0	58.1	2136 bp	1.0	78.6
ycf66_p4	ATCCTTTACCCCCAGTTCGA	AGGCCTTGATGGTGAAATGG	50.0	50.0	58.0	58.1	2101 bp	1.0	78.6
ycf66_p5	GGGTGTCGCCTCGAAAATAT	AGGCCTTGATGGTGAAATGG	50.0	50.0	58.1	58.1	2077 bp	1.0	78.5