Hotspot

ycf3

Genome · 35013-36869 · 1857 bp

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Pi 0

MarkerSeek score 58.10

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: Genome
length_bp: 1857
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	GGGAATATTAGAAATTTGCCAAAGC	AGGTGCCTAATGGACCGTAT	36.0	50.0	57.5	58.2	1973-1974 bp	1.0	40.0
ycf3_p2	GGGAATATTAGAAATTTGCCAAAGC	AAGGTGCCTAATGGACCGTA	36.0	50.0	57.5	58.4	1974-1975 bp	1.0	40.0
ycf3_p3	GGGAATATTAGAAATTTGCCAAAGC	TAAGGTGCCTAATGGACCGT	36.0	50.0	57.5	58.4	1975-1976 bp	1.0	40.0
ycf3_p4	GGGAATATTAGAAATTTGCCAAAGC	GGTGCCTAATGGACCGTATT	36.0	50.0	57.5	57.4	1972-1973 bp	1.0	40.0
ycf3_p5	GGGAATATTAGAAATTTGCCAAAGC	TTAAGGTGCCTAATGGACCG	36.0	50.0	57.5	57.3	1976-1977 bp	1.0	40.0