Hotspot

psbE

Genome · 18939-19190 · 252 bp

Back to Plagiomnium

Pi 0

MarkerSeek score 25.40

Primer available yes

Label psbE

Feature Metadata

label_name: psbE
feature_type: gene
region: Genome
length_bp: 252
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE_p1	AGCCTCTACAAATATATATGGAGCT	GGCCAACCACCTAACTGTAA	36.0	50.0	57.0	57.4	338 bp	1.0	40.0
psbE_p2	AGCCTCTACAAATATATATGGAGCT	TAGGTACAGCTAGTCCGTGC	36.0	55.0	57.0	58.6	360 bp	1.0	40.0
psbE_p3	ACACTGATAATCTAACTAGGCT	GGCCAACCACCTAACTGTAA	36.4	50.0	53.8	57.4	425-426 bp	1.0	40.0
psbE_p4	AGCCTCTACAAATATATATGGAGCT	TGTAGGTACAGCTAGTCCGT	36.0	50.0	57.0	57.2	362 bp	1.0	40.0
psbE_p5	ACACTGATAATCTAACTAGGCT	TAGGTACAGCTAGTCCGTGC	36.4	55.0	53.8	58.6	447-448 bp	1.0	40.0