Hotspot

petL

Genome · 17996-18091 · 96 bp

Back to Plagiomnium

Pi 0.0104

MarkerSeek score 58.50

Primer available yes

Label petL

Feature Metadata

label_name: petL
feature_type: gene
region: Genome
length_bp: 96
variable_sites: 1
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL_p1	TCGAGAAATACAATGAAATTAATCTGA	AGGTAATTGTTTTAGAAAAGATGT	25.9	25.0	55.8	52.9	212 bp	1.0	40.4
petL_p2	CGAGAAATACAATGAAATTAATCTGAC	AGGTAATTGTTTTAGAAAAGATGT	29.6	25.0	55.8	52.9	211 bp	1.0	40.4
petL_p3	ACAATGAAATTAATCTGACCTTAA	AGGTAATTGTTTTAGAAAAGATGT	25.0	25.0	52.7	52.9	203 bp	1.0	40.4
petL_p4	AGAAATACAATGAAATTAATCTGACCT	AGGTAATTGTTTTAGAAAAGATGT	25.9	25.0	55.7	52.9	209 bp	1.0	40.4
petL_p5	TGAAATTAATCTGACCTTAAAAGT	AGGTAATTGTTTTAGAAAAGATGT	25.0	25.0	52.6	52.9	199 bp	1.0	40.4