Hotspot

atpF

Genome · 65873-67104 · 1232 bp

Back to Plagiomnium

Pi 0
MarkerSeek score 58.90
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
Genome
length_bp
1232
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 TGCTAATTTCGTCAGGTCGA AACCTATTAATGGGAGAGAGA 45.0 38.1 57.0 52.7 1327-1329 bp 1.0 40.1
atpF_p2 TGCTAATTTCGTCAGGTCGA ACCTATTAATGGGAGAGAGAA 45.0 38.1 57.0 52.7 1326-1328 bp 1.0 40.1
atpF_p3 TGCTAATTTCGTCAGGTCGA AACCTATTAATGGGAGAGAGAA 45.0 36.4 57.0 53.7 1327-1329 bp 1.0 40.1
atpF_p4 CTGCTAATTTCGTCAGGTCGA AACCTATTAATGGGAGAGAGA 47.6 38.1 58.1 52.7 1328-1330 bp 1.0 40.1
atpF_p5 CTGCTAATTTCGTCAGGTCGA ACCTATTAATGGGAGAGAGAA 47.6 38.1 58.1 52.7 1327-1329 bp 1.0 40.1