Hotspot

accD

Genome · 24756-25685 · 930 bp

Back to Plagiomnium

Pi 0.00108

MarkerSeek score 59.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: Genome
length_bp: 930
variable_sites: 1
pi: 0.00108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GCTCTACCCAAAGCCATTCA	TGGATTAGAGCGCATGGTTC	50.0	50.0	57.9	58.0	1901 bp	1.0	78.6
accD_p2	GCTCTACCCAAAGCCATTCA	GATCTTGCTCGCGAAGGTAA	50.0	50.0	57.9	58.1	2248-2249 bp	1.0	78.4
accD_p3	GCTCTACCCAAAGCCATTCA	TGCTCGCGAAGGTAATGAAA	50.0	45.0	57.9	57.9	2243-2244 bp	1.0	78.3
accD_p4	GCTCTACCCAAAGCCATTCA	TTGCTCGCGAAGGTAATGAA	50.0	45.0	57.9	57.9	2244-2245 bp	1.0	78.3
accD_p5	GCTCTACCCAAAGCCATTCA	GGTGGTATCCATGTTTGGCA	50.0	50.0	57.9	58.2	2416-2417 bp	1.0	77.8