Hotspot

trnW-CCA-petG

Genome · 17628-17759 · 132 bp

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Pi 0

MarkerSeek score 33.80

Primer available yes

Label trnW-CCA-petG

Feature Metadata

label_name: trnW-CCA-petG
feature_type: igs
region: Genome
length_bp: 132
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 33.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-CCA-petG_p1	AAGCGCTCTTAGTTCAGTCC	GCTGGATCATTTGTAACCGC	50.0	50.0	57.6	57.5	268 bp	1.0	73.2
trnW-CCA-petG_p2	AGAACGCAGGTCTCCAAAAC	GCTGGATCATTTGTAACCGC	50.0	50.0	58.4	57.5	245 bp	1.0	73.0
trnW-CCA-petG_p3	GTAGAACGCAGGTCTCCAAA	GCTGGATCATTTGTAACCGC	50.0	50.0	57.5	57.5	247 bp	1.0	72.6
trnW-CCA-petG_p4	AAGCGCTCTTAGTTCAGTCC	TGGATCATTTGTAACCGCCT	50.0	45.0	57.6	57.2	266 bp	1.0	70.6
trnW-CCA-petG_p5	AGAACGCAGGTCTCCAAAAC	TGGATCATTTGTAACCGCCT	50.0	45.0	58.4	57.2	243 bp	1.0	70.4