Hotspot

rpl14-rps8

Genome · 5362-5698 · 337 bp

Back to Plagiomnium

Pi 0

MarkerSeek score 23.60

Primer available yes

Label rpl14-rps8

Feature Metadata

label_name: rpl14-rps8
feature_type: igs
region: Genome
length_bp: 337
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps8_p1	CCCTGTCGCACGAGAATTAA	CAAGTTCGCGTTCCTTATAT	50.0	40.0	58.0	53.5	461 bp	1.0	44.3
rpl14-rps8_p2	AAGGAACACGTGTTTTTGGC	CAAGTTCGCGTTCCTTATAT	45.0	40.0	57.7	53.5	480 bp	1.0	42.1
rpl14-rps8_p3	CCCTGTCGCACGAGAATTAA	CAAGTTCGCGTTCCTTATATATGT	50.0	37.5	58.0	57.2	461 bp	1.0	41.8
rpl14-rps8_p4	CCCTGTCGCACGAGAATTAA	AGTTCGCGTTCCTTATATATGT	50.0	36.4	58.0	55.2	459 bp	1.0	41.4
rpl14-rps8_p5	AAGGAACACGTGTTTTTGGC	CAAGTTCGCGTTCCTTATATATGT	45.0	37.5	57.7	57.2	480 bp	1.0	40.0