Hotspot

rps12

Genome · 103753-104583 · 831 bp

Back to Plagiogyria

Pi 0.00602

MarkerSeek score 62.20

Primer available yes

Label rps12

Feature Metadata

label_name: rps12
feature_type: gene
region: Genome
length_bp: 831
variable_sites: 5
pi: 0.00602
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12_p1	GAATGACGAGGAGCCGTATG	TTCTGTTCGCTACACTGCTC	55.0	50.0	58.2	57.9	1021 bp	1.0	78.0
rps12_p2	GGTGGGAATCTCATGTACGG	TTCTGTTCGCTACACTGCTC	55.0	50.0	57.8	57.9	1000 bp	1.0	77.9
rps12_p3	AATTGAATGACGAGGAGCCG	TTCTGTTCGCTACACTGCTC	50.0	50.0	58.3	57.9	1025 bp	1.0	77.1
rps12_p4	AATGACGAGGAGCCGTATGA	TTCTGTTCGCTACACTGCTC	50.0	50.0	58.6	57.9	1020 bp	1.0	75.1
rps12_p5	TGAATGACGAGGAGCCGTAT	TTCTGTTCGCTACACTGCTC	50.0	50.0	58.6	57.9	1022 bp	1.0	75.1