Hotspot

cemA

Genome · 64877-66382 · 1506 bp

Back to Plagiogyria

Pi 0.0106

MarkerSeek score 64.80

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 1506
variable_sites: 16
pi: 0.0106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	AGAGAAAGTGGGGAGAGTCC	GGATAAGTCCAGCCCTTTAACA	55.0	45.5	58.1	57.8	1641-1648 bp	1.0	62.8
cemA_p2	AGAAAGTGGGGAGAGTCCAA	GGATAAGTCCAGCCCTTTAACA	50.0	45.5	57.9	57.8	1639-1646 bp	1.0	62.3
cemA_p3	CGGATTCCCTCCTCCTGATA	GGATAAGTCCAGCCCTTTAACA	55.0	45.5	57.7	57.8	1572-1579 bp	1.0	60.8
cemA_p4	GGGGAGAGTCCAAAAGGGTA	GGATAAGTCCAGCCCTTTAACA	55.0	45.5	58.3	57.8	1632-1639 bp	1.0	60.6
cemA_p5	GAGAAAGTGGGGAGAGTCCA	GGATAAGTCCAGCCCTTTAACA	55.0	45.5	58.4	57.8	1640-1647 bp	1.0	60.4