Hotspot

rpl22-rps19

Genome · 88389-88405 · 17 bp

Back to Plagiogyria

Pi 0.0588

MarkerSeek score 74.30

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: Genome
length_bp: 17
variable_sites: 1
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TGATCGATAATCCGTCGCAC	ACTGATCGTATGGTGGGTCA	50.0	50.0	58.0	58.1	202 bp	1.0	78.9
rpl22-rps19_p2	TGATCGATAATCCGTCGCAC	TCGTATGGTGGGTCACAAAC	50.0	50.0	58.0	57.8	197 bp	1.0	78.7
rpl22-rps19_p3	TGATCGATAATCCGTCGCAC	CGTATGGTGGGTCACAAACT	50.0	50.0	58.0	57.8	196 bp	1.0	78.6
rpl22-rps19_p4	GTCGCACCTTAGTAGCTGAC	ACTGATCGTATGGTGGGTCA	55.0	50.0	58.1	58.1	189 bp	1.0	78.6
rpl22-rps19_p5	CGTACGAACAACCCTGGATT	ACTGATCGTATGGTGGGTCA	50.0	50.0	57.9	58.1	222 bp	1.0	78.5