Hotspot

trnD-GUC-psbM

Genome · 28099-34438 · 6340 bp

Back to Plagiogyria

Pi 0.0674

MarkerSeek score 70.40

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: Genome
length_bp: 6340
variable_sites: 426
pi: 0.0674
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	CTCATCAGCCAGGGGGTATA	GACGCCATATGCTCGATGAT	55.0	50.0	58.0	58.0	1218-1227 bp	1.0	87.9
trnD-GUC-psbM_p2	TACCAGCTGCCTCCTCTATC	GACGCCATATGCTCGATGAT	55.0	50.0	58.0	58.0	1075 bp	0.5	60.0
trnD-GUC-psbM_p3	CTATACCAGCTGCCTCCTCT	GACGCCATATGCTCGATGAT	55.0	50.0	58.0	58.0	1078 bp	0.5	59.9
trnD-GUC-psbM_p4	GTCCCTACGTATGCTTGCAA	GACGCCATATGCTCGATGAT	50.0	50.0	58.0	58.0	1491 bp	0.5	59.8
trnD-GUC-psbM_p5	TACCAGCTGCCTCCTCTATC	GGTGCTGGAGTGGTTGTTTA	55.0	50.0	58.0	58.0	731 bp	0.5	59.8