Hotspot

ycf12-trnG-UCC

Genome · 9198-9346 · 149 bp

Back to Plagiogyria

Pi 0.0403
MarkerSeek score 64.80
Primer available yes
Label ycf12-trnG-UCC

Feature Metadata

label_name
ycf12-trnG-UCC
feature_type
igs
region
Genome
length_bp
149
variable_sites
6
pi
0.0403
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf12-trnG-UCC_p1 AATAGTTGCATCGGGACCAC TACCACTAAACCATACCCGC 50.0 50.0 58.0 57.0 230-234 bp 1.0 73.9
ycf12-trnG-UCC_p2 AATTGCTCTATCGGCAGCTC TACCACTAAACCATACCCGC 50.0 50.0 58.1 57.0 206-210 bp 1.0 73.4
ycf12-trnG-UCC_p3 TGCATCGGGACCACTAGTAA TACCACTAAACCATACCCGC 50.0 50.0 58.1 57.0 224-228 bp 1.0 73.1
ycf12-trnG-UCC_p4 TTGCATCGGGACCACTAGTA TACCACTAAACCATACCCGC 50.0 50.0 58.1 57.0 225-229 bp 1.0 73.1
ycf12-trnG-UCC_p5 TAGTTGCATCGGGACCACTA TACCACTAAACCATACCCGC 50.0 50.0 58.1 57.0 228-232 bp 1.0 73.0