Hotspot

ycf1

SSC · 123709-129378 · 5670 bp

Back to Pittosporum

Pi 0.00146

MarkerSeek score 43.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5670
variable_sites: 51
pi: 0.00146
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAACTCTCGCTTTTTATACTCT	GAACCCATTCGTCTGTCTTT	34.8	45.0	55.2	55.7	339 bp	1.0	42.1
ycf1_p2	AAGAACTCTCGCTTTTTATACTCT	GAACCCATTCGTCTGTCTTT	33.3	45.0	55.9	55.7	340 bp	1.0	42.1
ycf1_p3	AGAACTCTCGCTTTTTATACTCTT	GAACCCATTCGTCTGTCTTT	33.3	45.0	55.9	55.7	339 bp	1.0	42.1
ycf1_p4	ACTCTCGCTTTTTATACTCTTTTCA	GAACCCATTCGTCTGTCTTT	32.0	45.0	56.8	55.7	336 bp	1.0	42.1
ycf1_p5	GAACTCTCGCTTTTTATACTCT	GAACCCATTCGTCTGTCTTT	36.4	45.0	53.7	55.7	338 bp	1.0	42.1