Hotspot

rps18

LSC · 68938-69243 · 306 bp

Back to Pittosporum

Pi 0.000871

MarkerSeek score 27.20

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 306
variable_sites: 2
pi: 0.000871
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	AGATCCGACCGAAATAGGAT	AATCTGCGTTTGAGTTCGGA	45.0	45.0	55.5	57.8	410 bp	0.866667	53.8
rps18_p2	AGATCCGACCGAAATAGGAT	TCTGCGTTTGAGTTCGGATT	45.0	45.0	55.5	57.8	408 bp	0.866667	53.8
rps18_p3	AGATCCGACCGAAATAGGAT	CAATCTGCGTTTGAGTTCGG	45.0	50.0	55.5	57.8	411 bp	0.866667	53.3
rps18_p4	AGATCCGACCGAAATAGGAT	CTGCGTTTGAGTTCGGATTG	45.0	50.0	55.5	57.8	407 bp	0.866667	53.3
rps18_p5	AGATCCGACCGAAATAGGATT	TCTGCGTTTGAGTTCGGATT	42.9	45.0	56.3	57.8	408 bp	0.866667	52.3