Hotspot

ndhD

SSC · 115531-117042 · 1512 bp

Back to Pittosporum

Pi 0.000794

MarkerSeek score 35.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1512
variable_sites: 9
pi: 0.000794
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACAAAGACCGAACATAGCGA	GGTTTTTCTGGTCCAACGTG	45.0	50.0	57.3	57.5	1651-1661 bp	0.933333	68.0
ndhD_p2	CACAAAGACCGAACATAGCG	GGTTTTTCTGGTCCAACGTG	50.0	50.0	57.2	57.5	1652-1662 bp	0.933333	67.6
ndhD_p3	ACAAAGACCGAACATAGCGA	ACGGGTTTTTCTGGTCCAAC	45.0	50.0	57.3	58.6	1654-1664 bp	0.933333	67.1
ndhD_p4	ACAAAGACCGAACATAGCGA	GGGTTTTTCTGGTCCAACGT	45.0	50.0	57.3	58.6	1652-1662 bp	0.933333	67.1
ndhD_p5	ACAAAGACCGAACATAGCGA	CACGGGTTTTTCTGGTCCAA	45.0	50.0	57.3	58.6	1655-1665 bp	0.933333	67.1