Hotspot

petA-psbJ

LSC · 63773-64801 · 1029 bp

Back to Pittosporum

Pi 0.00261

MarkerSeek score 56.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1029
variable_sites: 15
pi: 0.00261
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	1179-1182 bp	1.0	78.5
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	1175-1178 bp	1.0	77.6
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	57.8	58.3	1162-1165 bp	1.0	77.6
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	1178-1181 bp	1.0	77.6
petA-psbJ_p5	ATCCATTACGTGTCCAAGGC	TGGTTCATATTCCGGGTTGG	50.0	50.0	58.0	57.6	1180-1182 bp	0.933333	74.8