Hotspot

rps16-trnQ-UUG

LSC · 6113-7021 · 909 bp

Back to Pittosporum

Pi 0.0035

MarkerSeek score 51.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 909
variable_sites: 14
pi: 0.0035
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	982-1256 bp	1.0	79.3
rps16-trnQ-UUG_p2	CATCGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	1000-1274 bp	1.0	78.0
rps16-trnQ-UUG_p3	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	997-1271 bp	1.0	77.4
rps16-trnQ-UUG_p4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	996-1270 bp	1.0	77.4
rps16-trnQ-UUG_p5	CACATCGGATCGTGTCCTTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1002-1276 bp	1.0	76.8