Hotspot

trnK-UUU-rps16

LSC · 4183-5002 · 820 bp

Back to Pittosporum

Pi 0.0046

MarkerSeek score 61.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 18
pi: 0.0046
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGAATCCTTCGCTTCATCCA	TCCTTGAAAAAGGGGCTCAG	50.0	50.0	58.1	57.7	970-979 bp	1.0	79.2
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	TCCTTGAAAAAGGGGCTCAG	50.0	50.0	58.2	57.7	922-931 bp	1.0	78.3
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	TCCTTGAAAAAGGGGCTCAG	50.0	50.0	58.2	57.7	921-930 bp	1.0	78.3
trnK-UUU-rps16_p4	TCAGTCGTGGTCTTCCAAAC	TCCTTGAAAAAGGGGCTCAG	50.0	50.0	57.8	57.7	1007-1016 bp	1.0	77.7
trnK-UUU-rps16_p5	CAGTCGTGGTCTTCCAAACT	TCCTTGAAAAAGGGGCTCAG	50.0	50.0	57.8	57.7	1006-1015 bp	1.0	77.6