Hotspot

ndhD-psaC

IRa · 139692-139818 · 127 bp

Back to Pithecellobium

Pi 0.0551

MarkerSeek score 81.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: IRa
length_bp: 127
variable_sites: 7
pi: 0.0551
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CCGCGGATATTGGGAAAACT	AAACAACTCGAAGCATGGGT	50.0	45.0	58.3	58.0	211 bp	1.0	79.9
ndhD-psaC_p2	AGAAGATTAAGGAACCCGCG	AAACAACTCGAAGCATGGGT	50.0	45.0	57.7	58.0	226 bp	1.0	79.8
ndhD-psaC_p3	AGAAGATTAAGGAACCCGCG	GGCATGAAACAACTCGAAGC	50.0	50.0	57.7	58.0	232 bp	1.0	79.7
ndhD-psaC_p4	CCGCGGATATTGGGAAAACT	GGCATGAAACAACTCGAAGC	50.0	50.0	58.3	58.0	217 bp	1.0	79.7
ndhD-psaC_p5	TAAGGAACCCGCGGATATTG	AAACAACTCGAAGCATGGGT	50.0	45.0	57.5	58.0	219 bp	1.0	78.1