Hotspot

ccsA-ndhD

IRa · 137663-138194 · 532 bp

Back to Pithecellobium

Pi 0.0407

MarkerSeek score 76.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: IRa
length_bp: 532
variable_sites: 20
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGGATCAATCTATTAGGAATCGG	CAGTTGACAAGGTCGAAGCT	41.7	50.0	57.7	58.1	591-631 bp	1.0	51.2
ccsA-ndhD_p2	TGGGATCAATCTATTAGGAATCGG	TCAGTTGACAAGGTCGAAGC	41.7	50.0	57.7	58.1	592-632 bp	1.0	51.1
ccsA-ndhD_p3	GGAATCGGACTACATAGTTATGGT	CAGTTGACAAGGTCGAAGCT	41.7	50.0	57.7	58.1	575-615 bp	1.0	50.9
ccsA-ndhD_p4	GGAATCGGACTACATAGTTATGGT	TCAGTTGACAAGGTCGAAGC	41.7	50.0	57.7	58.1	576-616 bp	1.0	50.9
ccsA-ndhD_p5	TCGGACTACATAGTTATGGTTCA	CAGTTGACAAGGTCGAAGCT	39.1	50.0	56.6	58.1	571-611 bp	1.0	50.2