Hotspot

trnN-GUU-ycf1

IRb · 118197-118787 · 591 bp

Back to Pithecellobium

Pi 0.0727

MarkerSeek score 79.60

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 591
variable_sites: 40
pi: 0.0727
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	GCAAAAATGCCTTCCTCGTC	ATAGAGTCCGACCACAACGA	50.0	50.0	58.0	58.2	1053-1078 bp	1.0	85.2
trnN-GUU-ycf1_p2	GCAAAAATGCCTTCCTCGTC	CTTCTCGGTTGCTTCTTCCA	50.0	50.0	58.0	57.8	1137-1162 bp	1.0	84.8
trnN-GUU-ycf1_p3	GCAAAAATGCCTTCCTCGTC	AAAACAGGCAGTGGTGTTCT	50.0	45.0	58.0	57.9	1718-1743 bp	1.0	83.4
trnN-GUU-ycf1_p4	CGGGAGAATTGATGACTGCA	AAAACAGGCAGTGGTGTTCT	50.0	45.0	58.0	57.9	1664-1689 bp	1.0	83.4
trnN-GUU-ycf1_p5	GGCAGAATAGCAGAGCAAGT	AAAACAGGCAGTGGTGTTCT	50.0	45.0	58.0	57.9	1755-1780 bp	1.0	83.1